BelloCell-500P
- Productivity: up to 109 cell/bottle
- Designed for regular incubator and long-term cell culture
- Low shear stress, high oxygen transfer, and foam-free
- Non-pyrogenic, non-cytotoxic
- Pre-sterilized (gamma-irradiated) and ready-to-use
Trade Description
Product Feature
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High-density Cell Culture System for Research & Development
BelloCell-500P bottle is the alternative of BelloCell-500 for automatic medium recirculation during the cell culture. In addition, the upper tubing (inlet) and lower tubing (outlet) of BelloCell-500P connect to the external medium bottle (5 L) for medium replenishment. The fresh medium is gradually introduced in BelloCell-500P while old medium is displaced, driven by the peristaltic pump BelloFeeder with set perfusion time.
One BelloCell-500P bottle is equivalent to dozens of roller bottles. It is applicable in seeding, binding assay/ human proteome/new drug discovery, virus production, recombinant protein production/ monoclonal antibody production, transient transfection, and tissue engineering.
Check other products of the BelloCell family on the right for your specific application.
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Specification:
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BelloCell-500P |
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Function |
For culture of anchorage-dependent cells |
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Dimension |
100 (ψ) × 243 (h)mm |
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Working Volume |
500 ml |
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Material |
PETG, LDPE/EVA, PP |
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Cap Type |
Vented screw cap |
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Vent Filter |
0.22 μm PTFE membrane |
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Port |
Inlet: 10 cm silicon tubing with female luer Outlet: 10 cm silicone tubing with male luer |
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Carrier Quantity |
865 pc (±5%) BioNOC II, ~5.5 g |
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Growth Area |
>15,600 cm2 |
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Sterilization |
Gamma irradiated |
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Cell Line |
CHO, BHK, VERO, HEK-293, Sf-9, PK, Hi-5, HeLa, ST, 3T3, MDBK, C-127, MDCK, Huh7, RK-12, Hybridoma, Primary cell |
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Storage |
RT |
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Shelf Life |
2 years |
Principle:
The principle of BelloCell-500P is to immobilize cells onto the BioNOC II matrices packed in its upper chamber. By compressing and decompressing the bellow of BelloCelll-500P by BelloStage machine, the culture medium inside rises and descends, submerging and exposing matrices periodically. During aeration, cells attached to porous matrices are exposed to the air through a thin liquid film. In the submerging phase, cells are in direct contact with the fresh medium for up-taking nutrients while metabolic waste can be diffused as well as CO2 can be exhausted efficiently. The concept of the design is to solve problems (shear stress, limited oxygen supply, and low cell density) frequently occurring on the commercial models, and to simplify the operation.
Performance:
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BelloCell-500P |
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Output |
Up to 109 cells |
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Shear Stress |
Low |
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Foaming |
No |
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Cell Doubling Time |
Shorter |
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Direct Cell Count |
Yes |
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CPE Observation |
Yes |
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Cell Harvest |
Yes |
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Extra Medium Supply |
Yes |
Resource:
2. Introduction of BelloCell and BelloStage
3. All BelloCell Selection Guide
4. Reference
5. Q&A
Order Information:
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Product |
Cat. No. |
Availability |
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BelloCell-500P Bottle, 4 bt/cs |
BCB01000 |
In Stock |
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BelloCell Continuous System Complete Set Ø BelloStage-3000 Control Panel x 1 set Ø Control Box x 1 set Ø Forceps x 2 pc Ø CVD kit (50 ml) x 1 bt Ø Ball Screw Grease (SR-1) x 5 ml/bt Ø BelloFeeder-1400 Pump Set x 1 set Ø Tubing Complete Set x2 set Ø GlucCell Glucose Monitoring System x 1 set |
BCS07000 |
In Stock |
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BelloFeeder-1400 Pump Set, 1pc/set |
BCA01400 |
In Stock |
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Tubing Complete Set Ø Silicone Tubing Set x 1 set Ø PP Pump Head x 1 pc Ø GL45 Stainless Head Plate x 1 pc |
BCA01301 |
In Stock |
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Disposable Silicone Tubing Accessory, 5pc/bx |
BCA01302 |
In Stock |
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Disposable Silicone Tubing Set & Pump Head Ø Tubing Set x 1 set Ø PP Pump Head x 1 pc |
BCA01303 |
In Stock |
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GL45 Stainless Head Plate, 1pc/pk |
BCA02005 |
In Stock |
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Forceps , 25 cm, 1pc/pk |
BCA02000 |
In Stock |
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Crystal Violet Dye Nucleus Count Kit, 100 ml/bt |
BCA02001 |
In Stock |
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BelloCell Filtered Cap, 6 pc/cs |
BCA02003 |
In Stock |
