Home > Product > Cell Culture Bioreactor > TideCell HD Cell Culture System > TideXCell-002 Pro HD Cell Culture System

TideXCell-002 Pro HD Cell Culture System

TideXCell-002 Pro HD Cell Culture System is a bench-top, fixed-bed bioreactor utilizing an unique respiratory culture principle under low shear stress and high aeration capability. The system can culture cell from 6 x10¹⁰ in a small operation footprint, which is equivalent to conventional 75 L stir tank bioreactor.

Trade Description

Product Feature

Principle:

TideXCell HD Cell Culture System inherits the same patented principle of BelloCell systems. Packed with porous BioNOC II matrices, the matrix vessel functions as an artificial lung with a huge contact surface for nutrition and aeration for cell culture. The culture medium is periodically drawn from the external Mixing Bag into the matrix vessel to submerge the matrices and withdrawn from the vessel to expose the matrices, driven by the applied air pressure. Thus, cells entrapped inside the matrices are able to receive sufficient nutrients and oxygen, while metabolic wastes such as lactate and carbon dioxide can be efficiently carried away in a gentle manner. The concept of the design is to solve problems (shear stress, limited oxygen supply, and low cell density) frequently occurring on the commercial models, and to simplify the operation.

The culture medium in the external mixing bag, homogenized by different agitation methods, flows between the matrix vessel in the incubator and mixing bag by the switch-over of negative / positive air pressures, submerging the matrices where cells attach. As a result, the matrices are submerged and exposed to air periodically. During the submerging phase, fresh nutrients replenish, and metabolic wastes are diffused and then carried away. Meanwhile, the carbon dioxide is forced to exhaust. During aeration, clean air is introduced and contacts with the porous matrices through a thin liquid film. The oxygen transfer efficiency is maximized. The culture medium is re-mixed and tuning for pH/DO in the mixing bag.

Resource:

1. TideXCell brochure

2. Reference

3. Q&A

Hardware	TideXCell-002 High-Density Cell Culture System
Production scale	2 L Matrix Vessel (110 g BioNOC II carriers), and 10~100 L Mixing vessel with culture media depending on process requirement
Equivalent roller bottle (850 cm²)	400
Equivalent conventional batch system	75 L
Shear stress	Low
Foaming	No
Productivity	Up to 6 X 10¹⁰ cells

Product Description

Product Specification:

Base Unit
Weight	240 kg
Required space (W x H x D)	1250 x 1150 x 700mm
Power supply	220 VAC 50/60Hz
Housing	Outter 304 stainless steel
Working volume	1 ~ 5L, Using disposable or reusable matrix vessel 1L, 2 L matrix vessel (glass), 5L (PP)
Max. surface area for cell growth	31.2 m²
Operating temperature	18 ~ 27 °C
Maximum relative humidity	80% for temperatures up to 31 °C decreasing linearly to 50% relative humidity at 40 °C, non-condensing environment, non-condensing environment
TideMotion flow rate	800 ~ 1800 ml/min
Interfaces	- Ethernet for SCADA system - USB - Aux USB - Interface to TideXCell®-002 Pro Mixer PT-100 thermometric sensor - Interface to TideXCell®-002 Pro Mixer load cell - Interface to TideXCell®-002 Pro Mixer power - Interface to TideXCell®-002 Pro Mixer agitation signal - Interface to TideXCell®-002 Pro Mixer electric heating jacket - Interface to TideXCell®-002 Pro Mixer pH probe - Interface to TideXCell®-002 Pro Mixer DO probe - Alarm Relay
Ports	- Air In for air source - N₂ In for N₂ source - O₂ In for O₂ source - CO₂ In for CO₂ source - F.Cir-Out for force circulation function - Mix-Gas for gas supplement to TideXCell®-002 Pro Mixer
Peristaltic Pump Module
Quantity	4
Controller	Fixed speed (regulated on\|off)
Speed	100 rpm
Pump head	Watson Marlow 114, Fast Load pump head
Flow rates	MasterFlex L/S 25 Silicone tubing ID 3/16”: 75 ml/min MasterFlex L/S 16 Silicone tubing ID 1/8”: 45 ml/min
Process Control and Measurements
Temperature	- Temperature control with Heating coil - Temperature control for RT+8 ~ 45.0 °C - Display resolution 0.1°C - Heating power: 780 W
O₂Concentration	- O₂ Concentration control with Air and N₂ gas injection - O₂ Concentration control for 5%~20% - Display resolution 0.1°C
CO₂Concentration	- CO₂ Concentration control with CO₂ gas injection - CO₂ Concentration control for 0%~10% - Display resolution 0.1°C
Aeration Module
Gas inlet	- Gas pressure: 1.2 ~ 1.5 barg - Gases: dry, oil and dust-free - Gas inlet connections with quick couplers for 4 x 6 mm reinforced tubing
Gas outlet	- Gas pressure: max .1 barg - Gas outlet connections with quick couplers for 4 x 6 mm reinforced tubing
Aeration control	- Continuous and automatic aeration control for air, O₂, N₂ and CO₂ - Gas flow control units with mass flow meter: Total of four; one per gas source - Flow rates of the gas flow control units: Air, N₂, and CO₂: 0 ~ 10 L/min O₂: 0 ~ 5 L/min
Interface
Control	Siemens PLC
Display	Siemens HMI
Optional external unit	Adaptable to TideCell SCADA System and Cell Harvest System
Regulatory compliance	CE

Related Supplies