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TideCell-020 HD Cell Culture System

TideCell-020 HD Cell Culture System is a pilot scale, fixed-bed bioreactor utilizing an unique respiratory culture principle under low shear stress and high aeration capability. The system can culture cell from 6 x10¹¹ in a small operation footprint, which is equivalent to conventional 750 L stir tank bioreactor.

Trade Description

Product Feature

Easy-to-use, Reliable, Pilot Scale ( for 10¹¹ cell production)

Pilot scale bioreactor for mammalian cell culture
Fixed-bed system with 10~20 L matrices capacity, equivalent to 200 L convention stir tank bioreactor.
Autoclavable and single-use matrix vessel provides adaptation flexibilities while reducing user’s budget and shortening the timeline.
Reduce contamination risks of medium homogenization by contact-free pump based on magnetic levitation.
Accommodate users for process development in the vaccine industry, enabling quick transition to cGMP production.
SCADA software included (option)

TideCell-020 High-Density Cell Culture System (TideCell-020 System) is a fixed-bed bioreactor designed for the pilot scale production of cell and virus. The yield of cells can reach around 10¹¹, which is equivalent to 200 L conventional stir tank bioreactor.

With the modular design, TideCell-020 System presents simple installation, easy-to-use, durability, and uncomplicated maintenance, and thus exceeds traditional SIP bioreactors. The single-use mixing bag (100 L or 200 L), with bearingless pump, ensures low-shear and foam-free homogenization of medium. This feature, combined with a disposable medium supply chamber, help maintain pH/nutrient consistency from one cell batch to the next.

By using the program TideCommander-020, users can easily get access to the process control and data management during the run. Batch data, including weight, agitation, pH, DO, CO₂ concentration, temperature, medium feeding, etc, and related trend graphics are allowed to compile and compare. Based on data from the pilot tests, this helps quickly refine some parameters and pre-define the mass per batch for the future cGMP production. The logging data / event record and alarm functions are also available for the user to properly act, minimizing potential risks while up-scaling.

In addition, CESCO Bioengineering fully supports all necessary document packages to support validation. TideCell-020 System is the very best choice for the pilot plant studies, as well as economical mass production.

Principle:

TideCell inherits the same patented principle of BelloCell bottles. Packed with porous BioNOC II matrices, the matrix vessel functions as an artificial lung with a huge contact surface for nutrition and aeration for cell culture. The culture medium is periodically drawn from the external medium bag (Mixing Bag) into the matrix vessel to submerge the matrices and withdrawn from the vessel to expose the matrices, driven by the applied air pressure. Thus, cells entrapped inside the matrices are able to receive sufficient nutrients and oxygen, while metabolic wastes such as lactate and carbon dioxide can be efficiently carried away in a gentle manner. The concept of the design is to solve problems (shear stress, limited oxygen supply, and low cell density) frequently occurring on the commercial models, and to simplify the operation.

The culture medium in the external mixing bag, homogenized by different agitation methods, flows between the matrix vessel in the incubator and mixing bag by the switch-over of negative / positive air pressures, submerging the matrices where cells attach. As a result, the matrices are submerged and exposed to air periodically. During the submerging phase, fresh nutrients replenish, and metabolic wastes are diffused and then carried away. Meanwhile, the carbon dioxide is forced to exhaust. During aeration, clean air is introduced and contacts with the porous matrices through a thin liquid film. The oxygen transfer efficiency is maximized. The culture medium is re-mixed and tuning for pH/DO in the mixing bag.

Performance:

Hardware	TideCell-020 High-Density Cell Culture System
Production scale	20 L Matrix Vessel (1,000 g BioNOC II carriers), and 100~1000L Mixing bag with culture media depending on process requirement
Equivalent roller bottle (850 cm²)	4,000
Equivalent conventional batch system	750 L
Shear stress	Low
Foaming	No
Productivity	Up to 6 X 10¹¹ cells

Resource:

1. TideCell Brochure

2. Reference

3. Q&A

Product Description

Product Specification:

Base Unit
Weight	230 kg
Required space (W x H x D)	1250 x 1150 x 700mm
Power supply	220 VAC 50/60Hz
Housing	Outter 304 stainless steel
Working volume	10 ~ 30L, Using disposable or reusable matrix vessel
Operating temperature	18 ~ 27 °C
Maximum relative humidity	80% for temperatures up to 31 °C decreasing linearly to 50% relative humidity at 40 °C, non-condensing environment, non-condensing environment
TideMotion flow rate	3500 ~ 5000 ml/min
Interfaces	- Ethernet for SCADA system - USB - Aux USB - Interface to TideCell®-020 Pro Mixer PT-100 thermometric sensor - Interface to TideCell®-020 Pro Mixer load cell - Interface to TideCell®-020 Pro Mixer power - Interface to TideCell®-020 Pro Mixer agitation signal - Interface to TideCell®-020 Pro Mixer electric heating jacket - Interface to TideCell®-020 Pro Mixer pH probe - Interface to TideCell®-020 Pro Mixer DO probe - Alarm Relay
Ports	- Air In for air source - N₂ In for N₂ source - O₂ In for O₂ source - CO₂ In for CO₂ source - F.Cir-Out for force circulation function - Mix-Gas for gas supplement to TideCell®-020 Pro Mixer
Peristaltic Pump Module
Quantity	4
Controller	Fixed speed (regulated on\|off)
Speed	70 rpm 210 rpm
Flow rates	95~120 ml/min @3/16” ID 2,100~2,200 ml/min @5/16” ID
Process Control and Measurements
Temperature	- Temperature control with Heating coil - Temperature control for RT+8 ~ 45.0 °C - Display resolution 0.1°C - Heating power: 780 W
O₂Concentration	- O₂ Concentration control with Air and N₂ gas injection - O₂ Concentration control for 5%~20% - Display resolution 0.1°C
CO₂Concentration	- CO₂ Concentration control with CO₂ gas injection - CO₂ Concentration control for 0%~10% - Display resolution 0.1°C
Aeration Module
Gas inlet	- Gas pressure: 1.2 ~ 1.5 barg - Gases: dry, oil and dust-free - Gas inlet connections with quick couplers for 4 x 6 mm reinforced tubing
Gas outlet	- Gas pressure: max .1 barg - Gas outlet connections with quick couplers for 4 x 6 mm reinforced tubing
Aeration control	- Continuous and automatic aeration control for air, O₂, N₂ and CO₂ - Gas flow control units with mass flow meter: Total of four; one per gas source - Flow rates of the gas flow control units: Air, N₂, and CO₂: 0 ~ 10 L/min O₂: 0 ~ 5 L/min
Interface
Control	Siemens PLC
Display	Siemens HMI
Optional external unit	Adaptable to TideCell SCADA System and Cell Harvest System
Regulatory compliance	CE

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