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TideCell-100 HD Cell Culture System

TideCell-100 HD Cell Culture System is a production scale, fixed-bed bioreactor utilizing an unique respiratory culture principle under low shear stress and high aeration capability. The system can culture cell from 3 x 10¹² in a small operation footprint, which is equivalent to conventional 3,750 L stir tank bioreactor.

Trade Description

Product Feature

Compact, Reliable, Production Scale ( for 10¹² cell production)

Production scale bioreactor for mammalian cell culture
Fixed-bed system with 50~100 L matrices capacity, equivalent to 1,000 L~2,000 L convention stir tank bioreactor.
Reduce contamination risks of medium homogenization by contact-free pump based on magnetic levitation.
Regulatory documentation support to a GMP environment
SCADA software included (option)

TideCell-100 High-Density Cell Culture System (TideCell-100 System) is a fixed-bed bioreactor designed for the mass production of cell, protein and virus. The high surface-to-volume ratio leads to the significantly reduced production space. The inherent benefit of “TideCell mechanism” ensures that the process can behave as expected at the final scale without unavoidable risks (limit oxygen supply, uneven nutrient distribution, channel effect, etc). The output of cells can reach around 5×10¹², which is equivalent to 2,000 L conventional stir tank bioreactor.

Integrated with single-use consumables and industry - recognized components, TideCell-100 System simplifies the operation, maintenance, and parts replacement, and thus fast turnaround of production campaigns can be achieved. The single-use mixing bag (500 L or 1,000 L), with bearingless, magnetic pump, ensures efficient, low-shear stress, and foam-free mass transfer. This feature, combined with a sterile medium supply chamber, help maintain pH/nutrient consistency from one cell batch to the next.

The program TideCommander-100 allows the user to set up parameters (weight, agitation, pH, DO, CO₂ concentration, temperature, medium feeding, etc), and to show comparisons of various batches, trends within different periods, and pre-configured reports. The logging data / event record and alarm functions are also available with respect to the security issue, minimizing potential risks while manufacturing.

In addition, CESCO Bioengineering fully supports all necessary document packages to support validation. Documents for technical descriptions (drawings, accessory list, etc), qualification of the system (DQ, IQ, OQ, etc), standard operation procedures, traceable calibration certificate.

TideCell-100 System is the very best choice for large-scale cell and viral vaccine production.

Hardware	TideCell-100 High-Density Cell Culture System
Production scale	100 L Matrix Vessel (5,000 g BioNOC II carriers), 500~2,000L Mixing bag and mixing tank up to 5,000 tank with culture media depending on process requirement
Equivalent roller bottle (850 cm²)	20,000
Equivalent conventional batch system	3,750 L
Shear stress	Low
Foaming	No
Productivity	Up to 3 X 10¹² cells

Product Description

Principle:

TideCell HD Cell Culture System inherits the same patented principle of BelloCell bottles. Packed with porous BioNOC II matrices, the matrix vessel functions as an artificial lung with a huge contact surface for nutrition and aeration during cell culture. The culture medium is periodically drawn from the external Mixing Bag into the matrix vessel to submerge the matrices and withdrawn from the vessel to expose the matrices, driven by the applied air pressure. Thus, cells entrapped inside the matrices are able to receive sufficient nutrients and oxygen, while metabolic wastes such as lactate and carbon dioxide can be efficiently carried away in a gentle manner. The concept of the design is to solve problems (shear stress, limited oxygen supply, and low cell density) frequently occurring on the commercial models, and to simplify the operation.

The culture medium in the external mixing bag, homogenized by different agitation methods, flows between the matrix vessel in the incubator and mixing bag by the switch-over of negative / positive air pressures, submerging the matrices where cells attach. As a result, the matrices are submerged and exposed to air periodically. During the submerging phase, fresh nutrients replenish, and metabolic wastes are diffused and then carried away. Meanwhile, the carbon dioxide is forced to exhaust. During aeration, clean air is introduced and contacts with the porous matrices through a thin liquid film. The oxygen transfer efficiency is maximized. The culture medium is re-mixed and tuning for pH/DO in the mixing bag.

Specification:

Base Unit
Weight	300 kg
Required space (W x H x D)	1450 x 1500 x 1050mm
Power supply	220 VAC 50/60Hz
Housing	Outter 304 stainless steel
Working volume	50 ~ 100L, Using disposable or reusable matrix vessel
Operating temperature	18 ~ 27 °C
Maximum relative humidity	80% for temperatures up to 31 °C decreasing linearly to 50% relative humidity at 40 °C, non-condensing environment, non-condensing environment
TideMotion flow rate	20,000 ~ 30,000 ml/min
Interfaces	- Ethernet for SCADA system - USB - Aux USB - Interface to TideCell®-100 Pro Mixer PT-100 thermometric sensor - Interface to TideCell®-100 Pro Mixer load cell - Interface to TideCell®-100 Pro Mixer power - Interface to TideCell®-100 Pro Mixer agitation signal - Interface to TideCell®-100 Pro Mixer electric heating jacket - Interface to TideCell®-100 Pro Mixer pH probe - Interface to TideCell®-100 Pro Mixer DO probe - Alarm Relay
Ports	- Air In for air source - N₂ In for N₂ source - O₂ In for O₂ source - CO₂ In for CO₂ source - F.Cir-Out for force circulation function - Mix-Gas for gas supplement to TideCell®-010 Pro Mixer
Peristaltic Pump Module
Quantity	4
Controller	Fixed speed (regulated on\|off)
Speed	70 rpm 210 rpm
Flow rates	95~120 ml/min @3/16” ID 2,100~2,200 ml/min @5/16” ID
Process Control and Measurements
Temperature	- Temperature control with Heating coil - Temperature control for RT+8 ~ 45.0 °C - Display resolution 0.1°C - Heating power: 780 W
O₂Concentration	- O₂ Concentration control with Air and N₂ gas injection - O₂ Concentration control for 5%~20% - Display resolution 0.1°C
CO₂Concentration	- CO₂ Concentration control with CO₂ gas injection - CO₂ Concentration control for 0%~10% - Display resolution 0.1°C
Aeration Module
Gas inlet	- Gas pressure: 1.2 ~ 1.5 barg - Gases: dry, oil and dust-free - Gas inlet connections with quick couplers for 4 x 6 mm reinforced tubing
Gas outlet	- Gas pressure: max .1 barg - Gas outlet connections with quick couplers for 4 x 6 mm reinforced tubing
Aeration control	- Continuous and automatic aeration control for air, O₂, N₂ and CO₂ - Gas flow control units with mass flow meter: Total of four; one per gas source - Flow rates of the gas flow control units: Air, N₂, and CO₂: 0 ~ 10 L/min O₂: 0 ~ 5 L/min
Interface
Control	Siemens PLC
Display	Siemens HMI
Optional external unit	Adaptable to TideCell SCADA System and Cell Harvest System
Regulatory compliance	CE

Resource:

1. TideCell Brochure

2. Reference

3. TideCell brochure

4. Q&A

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