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TideCell Cell Harvest System

TideCell Cell Harvest System

TideCell Cell Harvest System (TCCHS) is specially designed for TideCell HD Cell Culture systems for cell recovery from the
cultured Matrix Vessel. The TCCHS is a powerful tool to harvest and transfer viable cells from large scale TideCell systems in a
close and automatic system. It can also be applied to harvest cell mass for biological production and any related applications.
Up to 1011 cells can be harvested within one to three hours operation. Entire cell harvest process is done in a close system and
could eliminate the risks of contamination.

Trade Description

Product Feature

Cell Harvest System
 
Automatic process of cell harvest
  •    Automatic control for cell trypsinization, washing, and harvesting in a closed system.
  •    Designed for TideCell 2 L, 10 L, and 20 L matrix vessels.
  •    Shaking speed, incubation time, washing cycles are adjustable.
 
Harvesting sufficient quantity of cells necessary for subsequent purification or for scale-up cell culture is labor-intensive yet critical task. To achieve higher consistency and efficiency for cell collection, it may require different maneuvers (longer trypsinization time, or relative gentle agitation) for multiple cell lines. However, the significant involvement of labor and various operation parameters applied may introduce variability to the manufacturing process or routine use.
 
TideCell Cell Harvest System is an automated workstation for cell detachment and collection in 2 L, 10 L, and 20 L TideCell matrix vessel. Cells can be harvested by the robotics from the matrix vessel, and proceed to the next application, for example, scale-up cell culture. The design for closed system operation could minimize the risk of contamination during the process. Large amounts of cells with high viability could be harvested within a short period of time and cell activities can be retained. The 6 peristaltic pumps with various reagent tanks, two gantry motors (shake and rotary motors) provide more control flexibility, and the user-friendly software allows the user to adapt a bench protocol, optimize the harvesting efficiency, and build up the standard procedures.
 
Parameters such as shake speed, rotation speed, incubation time, wash time, repeat cycles, and etc can be easily modified to fit the user’s specific demand.

Specification:
Cell Harvest System
Dimension
Controller: 110(w) x 80(d) x 169(h) cm
Main Console: 110(w) x 110(d) x 184(h) cm
Power
AC 220 V, 50/60 Hz
Adapted Matrix Vessel
2 L Matrix Vessel: 15.6 (di.) x 44.1 cm (h)
10 L Matrix Vessel: 29 (di.) x 48 cm (h)
20 L Matrix Vessel: 28 (di.)x 53 cm (h)
Peristaltic Pump
 for “Buffer”, “Enzyme”, “Inhibitor”, “Culture Medium”, “Harvest”, and “Waste”
Pump Rate
20~250 rpm ± 5 rpm
Rotation Rate
0~10 rpm ± 2 rpm
Rotation Period
0~999 sec ± 5 sec
Shake Rate
50~300 rpm ± 10 rpm
Shake Period
0~999 sec ± 5 sec
Touch Panel
10.4” color display
 
Principle:
The whole procedure is based on the following steps:
1.          Wash of the adherent cells with PBS (removal of serum-containing medium and cell debris)
2.          Dissociation of cells from matrices with trypsin-EDTA solution (disruption of cell-substratum and cell-cell interactions)
3.          Inactivation of trypsin using inhibitors (termination of proteolysis process)
4.          Dispersion and harvesting of cells into the harvest tank (passaging of cells)
5.          Exhaust of the waste
 
Performance:
 
Cell Harvest System
Output
Up to 1011 cell per batch run
Required Time
from 30 min to 2 h, depending on user’s protocol
 
Resource:

1.          TideCell Cell Harvest Brochure

 
      

Order Information:
Product
Cat. No.
Availability
TideCell Cell Harvest System, 1 set
TCA73000
Contact CESCO Bioengineering
TideCell Matrix Vessel 2 L (110 g BioNOC II ), 1pc/pk
TCV20110
Contact CESCO Bioengineering
TideCell Matrix Vessel 10 L (550 g BioNOC II ), 1pc/pk
TCV20550
Contact CESCO Bioengineering
TideCell Matrix Vessel 20 L (1,100 g BioNOC II ), 1pc/pk
TCV21100
Contact CESCO Bioengineering
Tubing Manifold for TideCell Cell Harvest System
BBA404AA
Contact CESCO Bioengineering
TideCell-002 High-Density Cell Culture System
TCS00020
Contact CESCO Bioengineering
TideCell-010 High-Density Cell Culture System
TCS00100
Contact CESCO Bioengineering
TideCell-020 High-Density Cell Culture System
TCS00200
Contact CESCO Bioengineering