BioNOC II Matrix
BioNOC II Matrix
BioNOC II is the leading cell culture carriers for pack-bed bioreactors mainly for high-density adherent cell culture. There have been over 15 publications and thousands of customers in the world, which makes BioNOC II one of the standard component for cell culture industry.
Trade Description
Product Feature
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BioNOC II Matrix
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Proprietary Matrices for High-Density Cell Culture
Productivity: up to 109 cells/g of BioNOC II
Growth area: 2,400 cm2/g of BioNOC II
Low lint, convenient for adherent cell growth for protein or viral vaccine production
Process control and quality assurance (cGMP guideline), full support of documentation
BioNOC II are the matrices for the growth of most anchorage dependent cells such as animal, mammalian, and insect cells in both serum-containing and serum-free media. With high S/V ratio (~160 cm-1) and great hydrophicility, BioNOCII enables cells to grow in 2D monolayer, or even in 3D mode, in a batch or perfusion culture type. In addition, cells can be easily sampled and examined for morphology and viability.
BioNOC II is ideally suited for BelloCell and TideCell bioreactors at a large scale with a relatively small footprint, and also supports other pack-bed bioreactors. It have been used for a variety of cell strains in either research or production application. |
Specification:
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BioNOC II Matrix
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Function
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For culture of adherent cells
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Material
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100% non-woven PET filament
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Dimension
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5 mm × 10 mm strip
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Surface Area / g
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2,400 cm2/g
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Capacity
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up to 109 cell/g of BioNOC II
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Default Packed Volume
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15 ml/g (5.5 g for BelloCell-500, 110 g for 2 L matrix vessel, 550 g for 10 L matrix vessel, 1,100 g for 20 L matrix vessel, 5,500 g for 100 L matrix vessel)
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Pore Size
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50 ~200 µm
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Porosity
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90~94%
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Endotoxin Test
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< 0.25 EU/ml
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Bioburden Test
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< 1 CFU / g
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Cytotoxicity Tested
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Yes, pyrogen-free
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Cell Line
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CHO, CHO-K1, rCHO-hIgO, rC-127-TPA, HEK-293, VERO, SF-9, Hi-5, BHK-21, rBHK-Factor VIII, HepG2, Hela, Huh 7, RK-13, ST, MDCK, MDBK, 3T3, MRC-5, CEF, Human foreskin fibroblast, human muscle skeleton cell, human mesenchymal cell, human embryonic stem cell, etc
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Regulation Guideline
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USP Class VI, USP <87>,<83>, ISO 10993-5
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Storage
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Room tempeature, dark area
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Shelf Life
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2 years
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Sterilization
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Tolerate autoclave (121 C), gamma irradiation (25 kGy), and EO sterilization
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Principle:
BioNOC II matrices are made from non-woven fabric composed of 100% PET filament according to cGMP guidelines. The fabric is specifically surface-treated to be hydrophilic and biocompatible. Homogeneous filament and high porosity / hydrophicility ensure the sufficient exchange of O2/CO2 and nutrient/waste, as well as improve the balanced growth of cells in the 3D folded matrices.
Performance:
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Output
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High-density growth of cell, up to 109 cell/g of BioNOC II
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Re-use
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Not recommended
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Adaptability
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Applicable to various packed-bed or fludized-bed bioreactors
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Requirement for Pre-treatment
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Sterilize before use
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Hydrophicility
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High
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Pack volume
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Up to 55 g per liter of container
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Cell Stain
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Cells can be stained with clear matrix
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Resource:
2. Reference
3. Q&A
Order Information:
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Product
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Cat. No.
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Availability
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BioNOC II Cell Culture Carrier (50 g/bottle)
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BNC00050
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In stock
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BioNOC II Cell Culture Carrier (250 g/bottle)
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BNC00250
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In stock
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BioNOC II Cell Culture Carrier (1,000 g/pk)
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BNC01000
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In stock
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BioNOC II Cell Culture Carrier (550 g/pk x 2 pk)
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BNC01100
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In stock
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